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Click here or scroll down to respond to this candidateCandidate's Name
Street Address Lamarque Street
Mandeville, Louisiana Street Address
PHONE NUMBER AVAILABLE
EMAIL AVAILABLE
PROFESSIONAL PROFILE
Medical research specialist with 14 years research experience. Exceptional time management and organizational skills. Exceptional ability to work independently on research and development projects. Proven record of making quick and accurate decisions while effectively coordinating and completing multiple tasks. Established team leader and staff development expert; lead by example. Well-developed communication and analytical skills; able to resolve practical problems effectively and diplomatically. Strong background in budget management with nearly 100 percent of proficiency in a nonprofit model including; purchasing, inventory and tracking of expenditures.
REPRESENTATIVE ACCOMPLISHMENTS
RESEARCH
As medical research specialist, successfully completed development of a molecular assay for quantification of plasma viral load. First author of a manuscript detailing the plasma viral load assay. Designed, conducted, troubleshot, and interpreted validation studies of molecular virology assays and techniques.
Secured an ongoing agreement from an outside contract laboratory to process plasma viral loads. Hosted a site visit for contract laboratory to audit the Real-Time PCR core lab and the in-house qPCR assay for quantification of plasma viral load. The contract resulted in increased outside revenue for the core lab and the university.
Competently completed development of a High Resolution Melt assay for genetic testing of the non-human primate colony. Designed, conducted, troubleshot, and interpreted validation studies of genetic testing and techniques.
Efficiently coordinate with Veterinarians and Veterinary Medicine s staff to start accepting non-human primate colony samples for a high-throughput blood screening assay resulting in a five-fold increase in sample load. Ensure samples are processed accordingly to establish standards. Assisted leadership in the development of a DNA banking core lab.
As core laboratory manager, oversee efficient laboratory operations, established reputation for training of faculty and staff gaining respect of leadership through extensive technical knowledge base. Increased productivity and expanded test capability of core laboratory through acquisition of cost-effective analytical equipment.
Effectively incorporated new equipment in the core lab increasing the technological resources of the Center. Competently transferred assay platforms and developed assay SOPs and establish standards.
Drafted and co-authored business plan for the Pathogen Detection and Quantification Core that now serves as a model for core laboratories at Tulane Primate Center. Elements of plan include accounting, budgeting and projections to justify operations.
RESEARCH EXPERIENCE
February 2006 TULANE NATIONAL PRIMATE RESEARCH CENTER, Covington, Louisiana
to Present Medical Research Specialist
Real-Time PCR Core Laboratory, Division of Microbiology
Supervise daily operations, standards, and all safety procedures of laboratory. Plan and prioritize work schedules. Supervise and train medical research technicians and new personnel; ensure staff capabilities to perform assigned molecular methods.
Expertly assist faculty, staff and students with experimental design, troubleshooting and the performance of Real-Time PCR and molecular techniques. Prepare, develop and modify protocols, SOP s and other technical documents. Maintain test procedures, quality control procedures, and quality assurance plans. Proficiently designed excel files for assay workflow and data analysis.
Competently perform qPCR to determine specific viral load copy number between control and experimental groups. Perform RT-PCR to determine specific gene expression between control and experimental groups analyzed by agarose gel electrophoresis. Extract and analyze Viral RNA and DNA from primate plasma samples, primate cells, and tissue samples. Design Real-Time assays and construct ssRNA and plasmid DNA standards. Expertly design primers and probes for classical PCR and qPCR.
Execute Open Array assays to determine specific gene expression between control and experimental groups. Perform dPCR assays on the open array platform to determine specific viral load copy number. Competent data analysis using Statminer, Expression suite and OpenArray Digital software for miRNA, plasma and tissue samples.
Designed and developed HRM assays to determine specific genetic background of non-human primates. Perform qPCR based microarray assays to determine specific gene expression between control and experimental groups. Perform ELISA and blood separation techniques. Perform in situ hybridization to determine specific gene expression between control and experimental groups.
Proficient control of a non-profit budget; billing for services rendered, purchasing, and tracking inventory. Collaborates with center s controller, Oracle programmer and administrative staff in development and management of core laboratory s budget and recharge rates.
Accomplished in the planning, laboratory design, stocking and operation of newly constructed core laboratory. Coordinate laboratory maintenance. Maintain lab equipment and validate lab instruments; QuantStudio 12K flex, 7900HT, epMotion 5075TMX, Bioanalyzer 2100 and Roche Magna Pure LC 2.0.
Coordinate guest speakers for technical seminars.
January 2004 LOUISIANA STATE UNIVERSITY HEALTH SCIENCES CENTER,
to January 2006 New Orleans, Louisiana
Research Associate
Department of Physiology
Research aim:
- Investigated mediated renal microvascular vasoconstrictor and vasodilator responses in angiotensin type 1 and 2 receptor utilizing a transgenic mouse model.
Competently extracted DNA for Classical PCR to genotype mouse pups containing transgenic gene visualized by agarose gel electrophoresis. Successfully extracted protein for Western Blot to determine specific protein expression between control and experimental groups. Performed analysis by acrylamide gel electrophoresis. Performed histological techniques, immunohistochemistry and in situ hybridization. Established and maintained multiple mouse breeding colonies providing transgenic experimental model for studies. Assisted in planning and execution of organ harvesting and tail biopsies.
Proficiently trained laboratory technicians and new personnel. Planned schedule. Collected, recorded, analyzed and evaluated data. Prepared, developed, and modified protocols, SOP s and other technical documents. Managed budget, purchasing, inventory and tracking of expenditures. Compiled reports of completed projects for publication in scientific journals. Coordinated laboratory maintenance.
February 2002 to TULANE UNIVERSITY HEALTH SCIENCES CENTER,
December 2003 New Orleans, Louisiana
Research Laboratory Technician
Tulane Hypertension and Renal Center of Excellence,
Department of Physiology (September 2003 to December 2003)
Research aim:
- Investigated mediated renal microvascular vasoconstrictor and vasodilator responses in angiotensin type 1 and 2 receptor utilizing a transgenic mouse model.
Research Laboratory Technician
Department of Experimental Medicine (February 2002 to September 2003)
Research aim:
- Investigated the application of hormone analogs as tumor specific cytotoxic agents involving prostate cancer treatments utilizing a nude mouse model.
Competently extracted RNA from rodent tumor samples for analysis. Performed RT-PCR to determine specific gene expression between control and experimental groups performing analysis by agarose gel electrophoresis. Extracted DNA for Classical PCR to genotype mouse pups containing transgenic gene visualized by agarose gel electrophoresis. Extracted protein for Western Blot to determine specific protein expression between control and experimental groups. Performed analysis by acrylamide gel electrophoresis.
Performed immunohistochemistry. Executed sterile, survival surgery in anesthetized mice for jugular venous injections and performed subcutaneous tumor implantation. Executed mice drug administration injection subcutaneous and intraperitoneal. Assisted in planning and execution of organ harvesting, autopsies and tail biopsies. Established and maintained multiple mouse breeding colonies providing transgenic experimental model for studies.
Assisted in the planning, design, stocking and operation of newly constructed core laboratory. Trained laboratory technicians and new personnel. Prepared, developed and modified protocols, SOP s and other technical documents. Planned schedule according to methods to be used, availability and quantity of resources. Collected, recorded, analyzed and evaluated data. Prepared reports for publication in scientific journals. Coordinated laboratory maintenance. Trained in cell culture techniques.
June 2000 LOUISIANA STATE UNIVERSITY SCHOOL OF DENTISTRY,
to August 2001 New Orleans, Louisiana
Student Intern
Department of Cell Biology and Anatomy
Research aim:
- Established a baseline of differentiation proteins in PDL and gingival fibroblast.
Successfully extracted RNA from clinical samples for analysis. Performed RT-PCR to determine specific gene expression between control and experimental groups analysis by agarose gel electrophoresis.
TRAINING
BSL-3 Biosafety Training, approved for escorted BSL-3 entry.
Tulane National Primate Research Center, Covington, LA. June 2010.
Hands on Real-Time PCR Course.
TATAA Biocenter, qPCR Symposium. San Francisco, CA. November 2009.
Principles of Quantitative and End-Point Applications for Real-Time PCR on the 7900. Applied Biosystems. Foster City, CA. July 2008.
Core Laboratory Management.
NIH. Rockville, MD. June 2006.
PERSONNEL TRAINED
Vibha Yadav, PostDoc Fellow,
Division of Microbiology, Tulane National Primate Research Center
John Karthik, PostDoc Fellow,
Division of Microbiology, Tulane National Primate Research Center
Uma Gautam, PostDoc Fellow,
Division of Bacteriology & Parasitology, Tulane National Primate Research Center Hong Ha, PostDoc Fellow,
Division of Pathology, Tulane National Primate Research Center
Wenpeng Li, PostDoc Fellow,
Division of Pathology, Tulane National Primate Research Center
Noton Dutta, PostDoc Fellow,
Division of Pathology, Tulane National Primate Research Center
Matthew Gilbert, PostDoc Fellow,
Division of Gene Therapy, Tulane National Primate Research Center
Eric Snook, PostDoc, NRSA Recipient,
Division of Gene Therapy, Tulane National Primate Research Center
Cynthia Ripoll, PostDoc, NRSA Recipient,
Division of Gene Therapy, Tulane National Primate Research Center
Mette Flaat, PhD Student,
Division of Gene Therapy, Tulane Health Sciences Center
Ji Yu, PhD Student,
Division of Gene Therapy, Tulane Health Sciences Center
Bindiya Patel, PhD Student,
Division of Gene Therapy, Tulane Health Sciences Center
Bonnie Phillips, PhD Student,
Division of Pathology, Tulane National Primate Research Center
Amanda McGillvray, PhD Student,
Division of Pathology, Tulane National Primate Research Center
Nell Bond, PhD Student,
Division of Microbiology, Tulane National Primate Research Center
Robert Cross, PhD Student,
Division of Microbiology, Tulane National Primate Research Center
Kaylie Tunik, PhD Student,
Division of Microbiology, Tulane National Primate Research Center
Yanhui Cai, PhD Student,
Division of Immunology, Tulane National Primate Research Center
Christine Robinson, Supervisor, Lab/Teacher,
Division of Gene Therapy, Tulane Health Sciences Center
Meredith Hunter, Supervisor, Lab/Instructor,
Division of Microbiology, Tulane National Primate Research Center
Stephanie Killeen, Supervisor, Lab/Teacher,
Division of Microbiology, Tulane National Primate Research Center
Coty Tatum, Medical Research Technician, Real-Time PCR Unit,
Division of Microbiology, Tulane National Primate Research Center
Surabhi Gaur, Medical Research Specialist,
Division of Microbiology, Tulane National Primate Research Center
Stephanie Freely, Medical Research Specialist,
Division of Microbiology, Tulane National Primate Research Center
Jeanne Macfarland, Medical Research Specialist,
Division of Microbiology, Tulane National Primate Research Center
Carys Kenway, Medical Research Technician,
Division of Pathology Tulane National Primate Research Center
Melissa Pattison, Medical Research Technician,
Division of Microbiology, Tulane National Primate Research Center
Chase Carter, Medical Research Technician, Real-Time PCR Unit,
Division of Microbiology, Tulane National Primate Research Center
Crystal Stoulig, Medical Research Technician,
Division of Pathology, Tulane National Primate Research Center
Kelly Golf, Medical Research Technician,
Division of Veterinary Medicine, Tulane National Primate Research Center
Jessica Eiermann, Medical Research Technician,
Division of Gene Therapy, Tulane National Primate Research Center
Nathan Ehrlich, Research Specialist III
University of Pittsburgh, Center for Vaccine Research
Daniel Mandell, Summer Student, Tulane University
Division of Microbiology, Tulane National Primate Research Center
Matthew Marx, Summer Student, Tulane University
Division of Microbiology, Tulane National Primate Research Center
Lauren Conroy, Summer Student, University of Notre Dame
Division of Microbiology, Tulane National Primate Research Center
LABORATORY SKILLS
Open Array dPCR HRM
qPCR RT-PCR, PCR ssRNA and DNA standard construction
Viral RNA Isolation RNA Isolation DNA Isolation
Poly-A RNA isolation in situ hybridization Immunohistochemistry
Western Blot ELISA Agarose gel Electrophoresis
Colony maintenance Small animal surgery Acrylamide gel Electrophoresis
Microscopy Spectroscopy Super-fusion
EDUCATION
Bachelor of Science: Biology, December 2001
University of New Orleans, Louisiana
PEER-REVIEWED PAPERS
C. J. Monjure, C. D. Tatum, A. T. Panganiban, M. Arainga, V. Traina-Dorge, P. A. Marx, Jr., E. S. Didier: Optimization of PCR for quantification of simian immunodeficiency virus (SIV) genomic RNA in plasma of rhesus macaques (Macaca mulatta) using armored RNA. J Primatology. 2014 Feb; 43(1): 31 43. doi: 10.1111/jmp.12088
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