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Click here or scroll down to respond to this candidateCURRICULUM VITAE
Candidate's Name , PhD
ADDRESS
Street Address
PHONE NUMBER AVAILABLE
EMAIL AVAILABLE
EDUCATION
1983-Ph.D. in Molecular Biology, Institute of Medical Genetics, Moscow, Russia.
Title of the Thesis: Molecular-Genetic Characteristic of alpha-Thalassemia.
1976-M.S. in Biochemistry, Moscow State University, Russia.
1971-1976-Moscow State University, Biological Department, School of Molecular Biology.
APPOINTMENTS
2014-present- Research Associate, University of New England
2004-2013- Research Associate, Thomas Jefferson University
2002-2004- Research Associate, Temple University
2001- 2002- Research Associate, Dupont Pharmaceuticals
1995-2001-Postdoctoral Fellowship, Kimmel Cancer Institute, Thomas Jefferson
University, and MCP Hahnemann University, Philadelphia.
1981-1994-Research Associate in the Laboratory of Molecular Genetics, Institute
of Medical Genetics, Moscow, Russia
AREA of CONCENTRATION
Anti-inflammatory effects of the Nigella sativa seed extract, thymoquinone, in pancreatic
cancer cells.
Induction of monocyte chemoattractant protein-1 by nicotine in pancreatic ductal adenocarcinoma
cells: role of osteopontin.
Induction of osteopontin expression by nicotine and cigarette smoke in the pancreas
and pancreatic ductal adenocarcinoma cells.
Vascular endothelial growth factor (VEGF) as a crucial proangiogenic component in
pancreatic ductal adenocarcinoma (PDAC).
The potential molecular basis for the role of AngII in islet inflammation through studying
its effect on MCP-1.
The cross talk between the IL-1beta and OPN pathways and a unique
trans-regulatory mechanism in which IL-1beta-induced NO synthesis feedback regulates
itself through up-regulation of OPN gene transcription in pancreatic islets.
Interaction between HIV-1 TAT protein and Rad51 recombinase. LTR activation by host
cellular proteins. Vpr, double stranded and end joining DNA repair.
Investigation of BRCA1 gene key role as a tumor suppressor in the genesis and progression
of breast and ovarian cancer.
Identification of the members of ets-oncogene superfamily, their structure, expression,
regulation and role in cellular transformation.
Study of molecular defects in the hereditary diseases: thalassemia, Isolated Growth
Hormone Deficiency, phenylketonuria.
TEACHING EXPERIENCE:
Teaching theory and sophisticated molecular biology research techniques to
graduate, postgraduate students and postdoctoral fellows, Thomas Jefferson University,
Medical school students, Jefferson Medical College,
Department of Surgery residents, Thomas Jefferson Hospital.
Development of research projects, performing research using state-of-the-art molecular,
serological and tissue culture techniques, working on data presentation for posters, oral
presentations and publications.
The ability to multi-task and manage several projects in parallel,
build a credible research environment within the laboratory with highest standards.
PROFESSIONAL SKILLS:
Strong methodological background in protein, RNA and DNA isolation, individual mRNA
purification, high molecular weight genomic DNA preparation.
Recombinant DNA Technique:
DNA cloning; PCR, RT-PCR, real time qPCR (Tagman, SYBR Green), QuantiGene Assay
(Panomics)
Southern hybridization analysis; Northern RNA hybridization;
Gene Function Analysis:
In vitro transcription and translation;
protein-protein interaction analysis (GST-pull down, immunoprecipitation); Western blot
assay; gel shift assay, chromatin immunoprecipitation (chip assay), Elisa.
DNA Repair Assays:
Homologous double stranded DNA repair and nonhomologous end joining.
Cell Biology:
Tissue culture in suspension and monolayer;
Transfection of mammalian cells including electroporation;
Silencing of gene expression with siRNA;
Infection with adenoviral particles and lentiviral sh RNA;
Beta-galactosidase detection in cell lysates and tissue, chloramphenicol acetyltransferase
and luciferase assays;
Stable clones selection;
MTT metabolic assay;
Soft agar growth assay;
Cell cycle analysis; fluorescence microscopy; immunohisto- and cytochemistry;
hybridization in situ;
Cell apoptosis: colorimetric caspASE assay system (Promega), TdT-FragEL DNA
Fragmentation Detection Assay(Calbiochem), AnnexinV-FITC apoptosis detection (BD
Pharmingen), DeadEnd Colorimetric TUNEL System (Promega).
Other Skills:
Mice injections; cryostat sectioning of tissue.
RECENT PUBLICATIONS:
Angiotensin II type 2 receptor: a novel modulator of inflammation in pancreatic ductal
adenocarcinoma through regulation of NFkB activity.
Ankit V. Gandhi, BS, Shivam Saxena, BS, Christopher Kang, MD, Konrad Sarosiek, M, Galina
Chipitsyna, PhD, Charles J. Yeo, MD, Hwyda A. Arafat, MD, PhD.
(Journal TBD)
Thymoquinone Promotes Pancreatic Cancer Cell Death and Reduction of Tumor Size through
Combined Inhibition of Histone Deacetylation and Induction of Histone Acetylation.
Daniel Relles, Candidate's Name , Qiaoke Gong, Hwyda A. Arafat.
(Journal of Advances in Preventive Medicine)
Hypothyroidism in Pancreatic Cancer: Role of Exogenous Thyroid Hormone in Tumor Invasion-
Preliminary Observations.
Sarosiek K, Gandhi AV, Saxena S, Kang CY, Chipitsyna GI, Yeo CJ, Arafat HA.
J Thyroid Res. 2016:2454989.
Conserved Metabolic Changes in Nondiabetic and Type 2 Diabetic Bariatric Surgery Patients:
Global Metabolomic Pilot Study.
Sarosiek K, Pappan KL, Gandhi AV, Saxena S, Kang CY, McMahon H, Chipitsyna GI,
Tichansky DS, Arafat HA.
J Diabetes Res. 2016:3467403.
Osteopontin (OPN) Isoforms, Diabetes, Obesity, and Cancer; What Is One Got to Do with the
Other? A New Role for OPN.
Sarosiek K, Jones E, Chipitsyna G, Al-Zoubi M, Kang C, Saxena S, Gandhi AV, Sendiky J, Yeo
CJ, Arafat HA.
J Gastrointest Surg. 2015 Jan 13.
Differential Expression of Cytochrome P450 Omega-hydroxylase Isoforms and Their Association
with Clinicopathological Features in Pancreatic Ductal Adenocarcinoma.
Gandhi AV, Saxena S, Relles D, Sarosiek K, Kang CY, Chipitsyna G, Andrel Sendecki J, Yeo CJ,
Arafat HA.
Annals of Surgical Oncology, 2013, July 12
Overexpressing TNF-Alpha in Pancreatic Ductal Adenocarcinoma Cells Modifies Cell Survival
and Reduces Fatty Acid Synthesis via Downregulation of Sterol Regulatory Element Binding
Protein-1 and Activation of Acetyl CoACarboxylate.
Mazhar Al-Zoubi, Candidate's Name , Konrad Sarosiek, Christopher Kang, Shivam Saxena,
Ankit Gandhi, Charles J Yeo and Hwyda A Arafat
Journal of Gastrointestinal Surgery, 2013, Oct. 4
Clinical Significance of Serum COL6A3 in Pancreatic Ductal Adenocarcinoma.
Kang CY, Wang J, Axell-House D, Soni P, Chu ML, Chipitsyna G, Sarosiek K, Sendecki J,
Hyslop T, Al-Zoubi M, Yeo CJ, Arafat HA.
Journal of Gastrointestinal Surgery, 2013, Sept. 4
RAN GTPase and Osteopontin in Pancreatic Cancer
Shivam Saxena, Ankit Gandhi, Pei-Wen Lim, Daniel Relles, Konrad Sarosiek, Christopher Kang,
Candidate's Name , Jocelyn Sendecki, Charles J Yeo and Hwyda A Arafat
Pancreatic Dis Ther 2013, 3:1
Circadian Gene Expression and Clinicopathologic Correlates in Pancreatic Cancer.
Relles D, Sendecki J, Chipitsyna G, Hyslop T, Yeo CJ, Arafat HA.
J Gastrointest Surg. 2012 Dec 20.
Serum monocyte chemoattractant protein-1 in pancreatic cancer.
Sullivan J, Gong Q, Hyslop T, Lavu H, Chipitsyna G, Yeo CJ, Arafat HA.
J Oncol. 2011: 518394. doi: 10.1155/2011/518394. Epub 2011 Oct 1.
Angiotensin II type 2 receptor blockade inhibits fatty acid synthase production through activation
of AMP-activated protein kinase in pancreatic cancer cells. McGhee A,
Sivarajah M, Gong Q, Lim S, Chipitsyna G, Yeo CJ, Arafat HA.
Surgery. 2011 Aug; 150(2):284-98. doi: 10.1016/j.surg.2011.06.002.
Tumor-specific expression and alternative splicing of the COL6A3 gene in pancreatic cancer.
Arafat H, Lazar M, Salem K, Chipitsyna G, Gong Q, Pan TC, Zhang RZ, Yeo CJ, Chu ML.
Surgery. 2011 Aug; 150(2):306-15. doi: 10.1016/j.surg.2011.05.011. Epub 2011 Jun 30.
Expression and regulation of nicotine receptor and osteopontin isoforms in human pancreatic
ductal adenocarcinoma.
Sullivan J, Blair L, Alnajar A, Aziz T, Chipitsyna G, Gong Q, Yeo CJ, Arafat HA.
Histol Histopathol. 2011 Jul; 26(7):893-904.
Expression and regulation of osteopontin in type 1 diabetes.
Gong Q, Chipitsyna G, Gray CF, Anandanadesan R, Arafat HA.
Islets. 2009 Jul-Aug; 1(1):34-41. doi: 10.4161/isl.1.1.8629.
Involvement of osteopontin in the matrix-degrading and proangiogenic changes mediated by
nicotine in pancreatic cancer cells.
Lazar M, Sullivan J, Chipitsyna G, Gong Q, Ng CY, Salem AF, Aziz T, Witkiewicz A, Denhardt
DT, Yeo CJ, Arafat HA.
J Gastrointest Surg. 2010 Oct; 14(10):1566-77. doi: 10.1007/sPHONE NUMBER AVAILABLE Epub 2010 Sep
8.
Induction of monocyte chemoattractant protein-1 by nicotine in pancreatic ductal adenocarcinoma
cells: role of osteopontin. Lazar M,
Sullivan J, Chipitsyna G, Aziz T, Salem AF, Gong Q, Witkiewicz A, Denhardt DT, Yeo CJ,
Arafat HA.
Surgery. 2010 Aug; 148(2):298-309. doi: 10.1016/j.surg.2010.05.002. Epub 2010 Jun 25.
Blocking angiotensin II Type 1 receptor triggers apoptotic cell death in human pancreatic cancer
cells.
Gong Q, Davis M, Chipitsyna G, Yeo CJ, Arafat HA.
Pancreas. 2010 Jul; 39(5):581-94. doi: 10.1097/MPA.0b013e3181c314cd.
Angiotensin II regulates the expression of monocyte chemoattractant protein-1 in pancreatic
cancer cells.
Chehl N, Gong Q, Chipitsyna G, Aziz T, Yeo CJ, Arafat HA.
J Gastrointest Surg. 2009 Dec; 13(12):2189-200. doi: 10.1007/sPHONE NUMBER AVAILABLE Epub 2009
Oct 9.
Anti-inflammatory effects of the Nigella sativa seed extract, thymoquinone, in pancreatic cancer
cells.
Chehl N, Chipitsyna G, Gong Q, Yeo CJ, Arafat HA.
HPB (Oxford). 2009 Aug; 11(5):373-81. doi: 10.1111/j.PHONE NUMBER AVAILABLEx.
Expression of a prometastatic splice variant of osteopontin, OPNC, in human pancreatic ductal
adenocarcinoma.
Sullivan J, Blair L, Alnajar A, Aziz T, Ng CY, Chipitsyna G, Gong Q, Witkiewicz A, Weber GF,
Denhardt DT, Yeo CJ, Arafat HA.
Surgery. 2009 Aug; 146(2):232-40. doi: 10.1016/j.surg.2009.03.036.
Induction of osteopontin expression by nicotine and cigarette smoke in the pancreas and
pancreatic ductal adenocarcinoma cells.
Chipitsyna G, Gong Q, Anandanadesan R, Alnajar A, Batra SK, Wittel UA, Cullen DM, Akhter
MP, Denhardt DT, Yeo CJ, Arafat HA.
Int J Cancer. 2009 Jul 15; 125(2):276-85. doi: 10.1002/ijc.24388.
Mitochondrial localization, ELK-1 transcriptional regulation and growth inhibitory functions of
BRCA1, BRCA1a, and BRCA1b proteins.
Maniccia AW, Lewis C, Begum N, Xu J, Cui J, Chipitsyna G, Aysola K, Reddy V, Bhat G,
Fujimura Y, Henderson B, Reddy ES, Rao VN.
J Cell Physiol. 2009 Jun; 219(3):634-41. doi: 10.1002/jcp.21708.
Angiotensin II induces vascular endothelial growth factor in pancreatic cancer cells through an
angiotensin II type 1 receptor and ERK1/2 signaling.
Anandanadesan R, Gong Q, Chipitsyna G, Witkiewicz A, Yeo CJ, Arafat HA.
J Gastrointest Surg. 2008 Jan; 12(1):57-66. Epub 2007 Nov 17.
Antihypertensives as novel antineoplastics: angiotensin-I-converting enzyme inhibitors and
angiotensin II type 1 receptor blockers in pancreatic ductal adenocarcinoma.
Arafat HA, Gong Q, Chipitsyna G, Rizvi A, Saa CT, Yeo CJ.
J Am Coll Surg. 2007 May; 204(5):996-1005; discussion 1005-6.
Induction of monocyte chemoattractant protein-1 expression by angiotensin II in the pancreatic
islets and beta-cells.
Chipitsyna G, Gong Q, Gray CF, Haroon Y, Kamer E, Arafat HA.
Endocrinology. 2007 May; 148(5):2198-208. Epub 2007 Feb 15.
Osteopontin protects the islets and beta-cells from interleukin-1 beta-mediated cytotoxicity
through negative feedback regulation of nitric oxide.
Arafat HA, Katakam AK, Chipitsyna G, Gong Q, Vancha AR, Gabbeta J, Dafoe DC.
Endocrinology. 2007 Feb; 148(2):575-84. Epub 2006 Nov 16.
Cooperativity between Rad51 and C/EBP family transcription factors modulates basal and Tat-
induced activation of the HIV-1 LTR in astrocytes.
Chipitsyna G, Sawaya BE, Khalili K, Amini S.
J Cell Physiol. 2006 Jun; 207(3):605-13.
Streptozotocin (STZ) mediates acute upregulation of serum and pancreatic osteopontin (OPN): a
novel islet-protective effect of OPN through inhibition of STZ-induced nitric oxide production.
Katakam AK, Chipitsyna G, Gong Q, Vancha AR, Gabbeta J, Arafat HA.
J Endocrinol. 2005 Nov; 187(2):237-47.
p27(SJ), a novel protein in St John's Wort, that suppresses expression of HIV-1 genome.
Darbinian-Sarkissian N, Darbinyan A, Otte J, Radhakrishnan S, Sawaya BE, Arzumanyan A,
Chipitsyna G, Popov Y, Rappaport J, Amini S, Khalili K.
Gene Ther. 2006 Feb; 13(4):288-95.
Mutations in a 23S rRNA gene of Chlamydia trachomatis associated with resistance to
macrolides.
Misyurina OY, Chipitsyna EV, Finashutina YP, Lazarev VN, Akopian TA, Savicheva AM,
Govorun VM.
Antimicrob Agents Chemother. 2004 Apr; 48(4):1347-9.
HIV-1 Tat increases cell survival in response to cisplatin by stimulating Rad51 gene expression.
Chipitsyna G, Slonina D, Siddiqui K, Peruzzi F, Skorski T, Reiss K, Sawaya BE, Khalili K,
Amini S. Oncogene. 2004 Apr 8; 23(15):2664-71.
c-Fos oncogene regulator Elk-1 interacts with BRCA1 splice variants BRCA1a/1b and enhances
BRCA1a/1b-mediated growth suppression in breast cancer cells.
Chai Y, Chipitsyna G, Cui J, Liao B, Liu S, Aysola K, Yezdani M, Reddy ES, Rao VN.
Oncogene. 2001 Mar 15; 20(11):1357-67.
REFERENCES:
Dr. Hwyda A. Arafat, MD, PhD:
Professor,
Department of Biomedical Sciences
420 Stella Maris Building
University of New England
College of Osteopathic Medicine
11 Hills Beach Rd.
Biddeford, ME 04005
PHONE NUMBER AVAILABLE
EMAIL AVAILABLE
Dr. Daniel Relles, MD
Fellow
Division of Pediatric Surgery
Morgan Stanley Childrens Hospital of New York Presbyterian
Columbia University College of Physicians and Surgeons
3959 Broadway, CHN 203
New York, NY 10032
Office: PHONE NUMBER AVAILABLE
EMAIL AVAILABLE
Dr. Konrad Sarosiek, MD
Department of Surgery
Jefferson Medical College
1015 Walnut Street
Suite 618 Curtis
Philadelphia, PA 19107
Office: PHONE NUMBER AVAILABLE
EMAIL AVAILABLE
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