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Title Research/Technology Development Assistant
Target Location CA-ON-Ottawa
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                                 Candidate's Name

Street Address  MacDonald Street, Halifax, NS B3L 3G5
PHONE NUMBER AVAILABLE   EMAIL AVAILABLE
LINKEDIN LINK AVAILABLE

                                                 SYNOPSIS
       Dalhousie University - Master of Science graduate (Street Address ), offering strong academic credentials

       and several years of experience in a multidisciplinary laboratory, biotechnology development,
       intellectual property development, and building/maintaining collaborations.
       Strong background in laboratory practices and techniques in biology, microbiology,
       biochemistry, and molecular biology; ability to quickly learn and master new technologies.

       Well-developed written and verbal communication skills; strong organizational and analytical
       skills; proficient in a range of computer programs including Microsoft Office Suite.

                                                E D U C AT I O N
         MASTER OF SCIENCE   Department of Biochemistry & Molecular Biology
         Dalhousie University, Halifax, Nova Scotia   April 2016


         BACHELOR OF SCIENCE   Major in Biology with Co-op option
         Acadia University, Wolfville, Nova Scotia   December 2013

                                                EXPERIENCE
         RESEARCH/TECHNOLOGY DEVELOPMENT ASSISTANT
              BIOCHEMISTRY & MOLECULAR BIOLOGY   HALIFAX, 2016 - PRESENT
                                                     SPRING
              NS
              (DALHOUSIE UNIVERSITY)
                  Responsible for developing spider silk technology including molecular construct

                  design, cloning (PCR, etc.), protein expression, protein purification, fibre production,
                  and downstream characterization; organized and managed all aspects of the project.
                  Managed grant-based and privately-funded research related to development and

                  commercialization of spider silk technology for various applications; responsible for
                  scaling-up E. coli silk protein production from shake-flask culture to bioreactor.
         GRADUATE RESEARCH ASSISTANT (MASTER S STUDENT)
              BIOCHEMISTRY & MOLECULAR BIOLOGY   HALIFAX, SUMMER 2014 - SPRING 2016
              NS
              (DALHOUSIE UNIVERSITY)
                  Successfully developed/optimized fibre production for recombinant spider wrapping

                  silk; produced proteins using E. coli and purified by IMAC; carried out protein analysis
                  by UV/Vis and SDS-PAGE; characterized fibre biophysical, mechanical, and
                  morphological (by AFM, SEM, and polarized light microscopy) properties.

                                                                                                 1|Page
                 Work resulted in primary authorship of a published peer-reviewed article, alongside

                 preparation of additional manuscripts; presented work at various conferences;
                 contributed to intellectual property development; built/maintained collaborations.
                                 Candidate's Name


PHONE NUMBER AVAILABLE   EMAIL AVAILABLE
LINKEDIN LINK AVAILABLE
                                            E X P E R I E N C E c ontinued

              BIOCHEMISTRY & MOLECULAR BIOLOGY   SAINT JOHN, 2014
                                                        WINTER
              NB
              (DALHOUSIE MEDICINE NEW BRUNSWICK)
                 Investigated enzymes in the murine heart; maintained and cultivated cell cultures;

                 conducted cloning/plasmid preparation; transfected cells for recombinant protein
                 expression; carried out sample analysis by BCA, SDS-PAGE, and Western blotting.
                 Mentored and supervised an undergraduate summer student; developed strong

                 analytical and problem-solving skills; developed organizational skills and flexibility in
                 a fast paced environment with changing priorities.
        LABORATORY TEACHING ASSISTANT
              NUCLEIC ACID BIOCHEMISTRY & MOLECULAR BIOLOGY                     FALL 2014/2015
              HALIFAX, NS
              (DALHOUSIE UNIVERSITY)
              INTRODUCTORY BIOCHEMISTRY LABORATORY   HALIFAX, INTER 2015
                                                           W
              NS
              (DALHOUSIE UNIVERSITY)
              INTRODUCTORY CHEMISTRY LABORATORY   SAINT JOHN,                           WINTER 2014
              NB
              (UNIVERSITY OF NEW BRUNSWICK SAINT JOHN)

        UNDERGRADUATE RESEARCH ASSISTANT (CO-OP STUDENT)
              BIOCHEMISTRY & MOLECULAR BIOLOGY   HALIFAX, NS                    SUMMER 2013
              (DALHOUSIE UNIVERSITY)
                 Iteratively re-designed peptides to further investigate self-assembling properties;

                 produced, purified, and characterized peptides using various analytical techniques;
                 work resulted in a manuscript in preparation for potential publication.

              BIOCHEMISTRY & MOLECULAR BIOLOGY   HALIFAX, INTER - SUMMER 2012
                                                       W
              NS
              (DALHOUSIE UNIVERSITY)
                 Used solid-phase peptide synthesis methods to produce peptide sequences with self-

                 assembly properties; purified peptides by HPLC; determined product masses and
                 sequences of peptides by MALDI- or ESI-mass spectrometry.
                 Characterized each peptide s suitability as a functional biomaterial using microscopy

                 techniques, far-UV circular dichroism spectroscopy, and fluorescence spectroscopy.

              WILDLIFE BIOLOGY   WOLFVILLE, NS                                  SUMMER 2011
                                                                                                2|Page
              (ACADIA UNIVERSITY/NOVA SCOTIA DNR)
                 Performed over 230 coyote necropsies and organ flushes to sample worms; recorded,

                 organized, and analyzed data using standard statistical methods; ensured accuracy of
                 data through careful collection and entry.
                 Work resulted in co-authorship of a published peer-reviewed article.


                                 Candidate's Name


PHONE NUMBER AVAILABLE   EMAIL AVAILABLE
LINKEDIN LINK AVAILABLE
                                               P U B L I C AT I O N S
         Shin, K., Chapman, N.A., Sarker, M., Kenward, K.A., Huang, S.K., Weatherbee-Martin,

         N., Pandey, A., Dupr , D.J., and Rainey, J.K. Bioactivity of the putative apelin proprotein
         expands the repertoire of apelin receptor ligands. (2017) Biochimica et Biophysica Acta -
         General Subjects. Under review.
         Weatherbee-Martin, N., Xu, L., Hupe, A., Kreplak, L., Fudge, D.S., Xiang-Qin, L., and Rainey,

         J.K. Identification of wet-spinning and post-spin stretching methods amenable to recombinant
         spider aciniform silk. (2016) Biomacromolecules 17(8): 2737 2746.
         Power, J., Weatherbee-Martin, N., Boudreau, M., O Brien, M., Conboy, G., and Smith, T.

         Diversity and ecology of pulmonary metastrongyloidosis in coyotes (Canis latrans) of Nova
         Scotia, Canada. (2015) Comparative Parasitology 82(1): 85-93.

                                              P R E S E N TAT I O N S
       Weatherbee-Martin, N., Xu, L., Hupe, A., Fudge, D.S., Kreplak, L., Liu, X-Q., and Rainey, J.K.
       (2016) Optimizing structural and mechanical properties of recombinant spider wrapping silk
       fibres. Institute for Research in Materials Annual General Meeting and Research Day 2016,
       organized by Dalhousie University - Halifax, NS. (Poster presentation; best poster prize).
       Weatherbee-Martin, N., Xu, L., Liu, X-Q., and Rainey, J.K. (2016) Automated spinning of
       fibrous recombinant spider wrapping silk. 99th Canadian Chemistry Conference and Exhibition
       - Halifax, NS. (Oral presentation).
       Weatherbee-Martin, N., Xu, L., Hupe, A., Fudge, D.S., Kreplak, L., Liu, X-Q., and Rainey, J.K.
       (2016) Optimizing structural and mechanical properties of recombinant spider wrapping silk
       fibres. 10th World Biomaterials Congress - Montr al, QC. (Poster presentation).
       Weatherbee-Martin, N., Xu, L., Liu, X-Q., and Rainey, J.K. (2015) Optimizing structural and
       mechanical properties of recombinant spider wrapping silk fibres. Graduate Student Research
       Day 2015, organized by IHRTP - Halifax, NS. (Poster presentation).

                                                   P AT E N T S
         Liu, Paul. 2016. Artificial spider aciniform silk proteins, methods of making and uses thereof.

         U.S. Patent Application No. 62287564, filed January 2016. Patent Pending.




                                            GRANTS            A WA R D S
                                                       AND

       IDEA TO INNOVATION GRANT                                               WINTER 2017
        o NATURAL SCIENCES AND ENGINEERING RESEARCH COUNCIL OF CANADA (NSERC)

                                                                                                3|Page
  PROOF OF CONCEPT AWARD
  SUMMER 2016
   o SPRINGBOARD INNOVATION MOBILIZATION PROGRAM
  PATENT & LEGAL AND PROOF OF CONCEPT AWARD                                  FALL
  2015
   o SPRINGBOARD INNOVATION MOBILIZATION PROGRAM
  ALEXANDER GRAHAM BELL CANADA GRADUATE SCHOLARSHIP-MASTER S
  SPRING 2015
   o NATURAL SCIENCES AND ENGINEERING RESEARCH COUNCIL OF CANADA (NSERC)




                                                                           4|Page

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