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Street Address Genesse Creek San Antonio, Texas Street Address
Cell Phone: PHONE NUMBER AVAILABLE E-mail: EMAIL AVAILABLEEDUCATION: University of Texas Health Science Center, San Antonio, TX M.S. Forensic Toxicology (Thesis-Based) May 20, 2007 Schreiner University, Kerrville, TX B.S. Biochemistry May 5, 2002 Relevant coursework: Biochemistry Genetics Microbiology Organic Chemistry Cell Biology MedicalTerminology Physical Chemistry Analytical Chemistry Anatomy &Physiology Forensic Toxicology Clinical ChemistryPharmacology Honors/Awards: Chancellors List for Graduate School, 2004-2006 President's List and Dean's List every semester Hill Country Scholarship, 2000-2002 Johnny R. Davis Scholarship, 1998-2002 Outstanding Workstudy, 2002WORK EXPERIENCE:Lab Manager/Toxicologist: LSASA, LLC, San Antonio, TX, May 2015-October2016.Duties: Responsible for technical and scientific oversight of thelaboratory. > Selects test methodologies appropriate for the clinical use of the test menu > Verifies test procedures performed and establishes the laboratory's performance criteria, including accuracy and precision of each test and test system > Enrolls the laboratory in an approved PT program commensurate with services offered > Establishes a quality control program appropriate for the testing performed > Establishes the acceptable levels of analytic performance, and ensures these levels are maintained throughout the testing process > Resolves technical problems and ensures remedial actions are taken whenever test systems deviate from the laboratory's established performance specifications > Ensures patient test results are not reported until all corrective action has been taken and the test system is functioning properly > Identifies training needs and ensures testing personnel receive regular in-service training > Evaluates the competency of all testing personnel on an ongoing basis > Evaluates and documents performance of individuals responsible for testing at six months and twelve months in the first year of employment and yearly thereafter, unless test methodology or instrumentation changes, in which case, prior to reporting patient test results, the individual's performance must be reevaluated for the new test methodology or instrumentation > Answers doctors/medical assistant's questions concerning drugs and metabolites and/or general toxicology questions > Correspond with sales reps on incorporating new tests and educating them on toxicology and drug metabolism > Toxicology sales and support for pain management, family practice and treatment centers.Lab Manager/Toxicologist: Frontier Toxicology, San Antonio, TX, February2014-April 2015.Duties: Responsible for technical and scientific oversight of thelaboratory, answering doctor's phone calls in regards to patient toxicologyresults and overall management of the laboratory.Lab Manager (Pharmacogenomics Lab): Frontier Toxicology, San Antonio, TX,January 2014-September 2014.Duties: Responsible for technical and scientific oversight of thelaboratory > Started up the Pharmacogenomic (PGx) laboratory at Frontier Toxicology > Review reports and communicate with doctors about drug metabolism and drug-drug interactionsAnalyst II/Toxicologist: Hill Country Toxicology, San Antonio, TX, May2013-January 2014.Duties: Performs laboratory tests, procedures, experiments and analyses toprovide data as specified or required in toxicological evaluation ofpatient samples. Communicated patient results and interpretations oftoxicological findings to doctors and/or medical staff. Reviewed all datafor QA/QC issues.Toxicology Chemist: Bexar County Medical Examiner's Office, San Antonio,TX, August 2008-May 2013.Duties: Responsible for performing toxicological analyses on cases submitted to the Medical examiners office and other functions as required by the QA Officer, a Senior Toxicology Chemist or the Chief Toxicologist.Research Assistant: Department of Pediatrics, Division of Neonatology,University of Texas Health Science Center, San Antonio, TX, September 2002-August 2008.Duties: Carry out molecular and biological techniques, such as, Western andNorthern blots, cell culture, protein assays, isolation and quantitation ofRNA, immunofluorescence, protein from whole tissue and cells, bacterialtransformations, transfections, and RT-PCR. Assist several pediatricfellows and neonatologists with laboratory and clinical based researchprojects.Work-Study Program: Biology Department, Schreiner University, Kerrville,TX, August 1998-May 2002.Duties: To help set up science labs, prepare solutions, clean glassware,inventory of chemicals, and assist students in lab processes.Alpha Diagnostics Int., Inc: Research/Sales Company, San Antonio, TX,Summer 2000 and 2001.Duties: Antibody analysis by ELISA assay, coupling proteins to sepharose,affinity purification of antibodies, and electrophoresis of brain anddigestive proteins.INTERNSHIP:Forensic Toxicology Lab: Bexar County Forensic Toxicology Lab, San Antonio,TX, Summer 2006.Duties: Analyzed drugs and poisons in human tissue and body fluids,including blood alcohol results. Performed acid/neutral and alkaline druganalysis and screening using GC and GC/MS, as well as, cocaine/opiates,THC, enzymatic assays, alcohols, accessioning, and Conway diffusion.SKILLS: LABORATORY: Chromatography: paper and thin layer Bacterial culturing Electrophoresis: SDS-PAGE and agarose Autoclaving Spectroscopy Micropipeting Purification of antibodies ELISA assays Protein Assays Cell Culture DNA/RNA Tissue explants Animal Handling/Surgery Microscopy Immunofluorescence Bacterial Transformations Prepare solutions/media Transfections GC/FID and GC/MSD RT-PCR TOXI-LAB LC/MS/MS Head-Space GC QA/QC Verification COMPUTERS: Microsoft Office, Spreadsheet Applications, Computer programming (Visual Basic), Front Page, Printshop Pro, Website creation, and Internet experience. OTHER: IRB writing and documentation procedures (based on clinical research) Writing/Reviewing SOP's Compliance protocols/guidelines QA/QC guidelinesACTIVITIES:Science Club (1999-2002)Science Club President (2001-2002)Expanding Your Horizons (1998-2003)Schreiner University Science Camp (1999, 2000)Student Senate Science Club Representative (2001-2002)Texas Academy of Science (1999-2002)UTHSCSA Safety Evacuation Committee (2005-2008)Southwestern Association of Toxicologists (SAT) Fall 2008 Meeting, Austin,TX (December 2008)SAT Spring 2009 Meeting, San Antonio, TX (April-May 2009)SAT Spring 2012 Meeting, San Antonio, TX (May 2012)PROFESSIONAL ASSOCIATION MEMBERSHIPS:Texas Academy of Science member (1999-2002)American Association of University Women (2002-2003)SAT Member (2009-present)SAT Vendor Liaison (2012-2014)TEACHING:2014-present Adjunct Assistant Professor, UTHSCSA, Clinical Laboratory Sciences, School of Health ProfessionsNeonatal Subspecialty Residents2002-2005 Dr. Johnson Isaac, M.D., Wilford Hall Medical Center. Finalist for The Young Investigator Award in Basic Science, The Southern Society for Pediatric Research, 2005. Manuscript titled "Mechanical stretch upregulates ?-epithelial Na+ channel (?- ENaC) expression in murine lung epithelial (MLE-12) cells via MAPK activation." To be Submitted.2004-2005 Dr. Ryan McAdams, M.D., Wilford Hall Medical Center. Manuscript titled "Cyclic stretch attenuates the effects of hyperoxia on cell proliferation and viability in human alveolar epithelial cells". American Journal of Physiology, Lung Cellular and Molecular Physiology, 291, L166-L174, 2006.2003-2006 Dr. Margarita Vasquez, M.D., UTHSCSA. Finalist for The Young Investigator Award in Basic Science, The Southern Society for Pediatric Research, 2006. Manuscript titled "Induction of serum and glucocorticoid-induced protein kinase-1 (sgk-1) and epithelial sodium channels (ENaC) by cAMP in epithelial cells".2004-2007 Dr. Michael Shoemaker, Ph.D., M.D., Wilford Hall Medical Center. Project title "Effects of hyperoxia on ENaC expression in murine lung epithelial cells".2005-2008 Dr. Chris Bishop, M.D., UTHSCSA. Project title "The Effects of IL-1 beta on Glucocorticoid Receptor Expression in MLE-12 Cells".2005-2008 Dr. Tim Biela, M.D., UTHSCSA. Project title "Regulation of Epithelial Sodium Channels by Synthetic Glucocorticoids in MLE- 12 Cells".2007-2008 Dr. Amy Fowler, M.D., UTHSCSA.2007-2008 Dr. Aaron Reeves, M.D., UTHSCSA. Project title "Trafficking and Function of the Epithelial Sodium Channel Is Inhibited By Epidermal Growth Factor In A Rat Parotid Gland Cell Line".2007-2008 Dr. Taslim Allibhai, M.D., Wilford Hall Medical Center.2007-2008 Dr. Kelsey Sherburne, M.D., UTHSCSA. Project title "Maternal Human Milk versus Donor Human Milk: an in vitro study on cytokine production and lactoferrin levels".2007-2008 Dr. Tania Hernandez, M.D., UTHSCSA. Project title "IL-1Transiently Increases Epithelial Sodium Channel Expression in Lung Epithelial Cells.Graduate2015 Salina Vargas, UTHSCA, MS candidate.2014 Richard Mazuca, UTHSCA, MS candidate. Project title "TheColorectal Carcinogenic Potential of Ingested Aloe barbadensis Miller'sAnthraquinones and Anthrones.2013 Victor Trejo, UTHSCA, MS candidate. Project title"Physiological similarities between the use of Synthetic Stimulants "Bath Salts" Cocaine, Ecstasy, and Methamphetamine.2008 Madhu Jupelli, UTSA, Ph.D. candidate. Project title "The mechanisms involved in development of airway hyper-reactivity in adult mouse following neonatal pulmonary Chlamydia infection".Undergraduate2003-2004 Haleema Harhara, St. Mary's University, volunteer summer student.2004 Supervision and teaching Christa Niemoller-Kincaid as part of an internship in collaboration with Schreiner University, TX.2005 Sarah Yoder, Rice University, volunteer summer student.2005 Ashaini Kadakia, UT Austin, volunteer summer student.2005-2006 Thesis Mentor for Senior UTSA Honors Student: Ana Nicho. Project title "Disruption of the cytoskeletal network alters Epithelial Na+ Channel (ENaC) activity".2006 Aasya Nasar, UT Austin, volunteer summer student.2006-2007 Jonathan Rodriguez, UTSA, volunteer.High School2002-2003 Mentor for a Health Careers High School Student as part of The Independent Student Mentorship Program: Kinza Shamsi, Project title "The Effect of Cytokines on Sodium Channel Protein Expression in Mouse Lung Epithelial Cells."2003 Aasya Nasar, volunteer summer student.2004 Ryan DiGeronimo, volunteer summer student.2005-2006 Mentor for a Health Careers High School Student as part of The Independent Student Mentorship Program: Chelsea Rodriguez, Project title "Research in the Division of Neonatology."PUBLICATIONS:Abstracts1. J.A. Petershack, S.R. Seidner, B.M. Henson, and S.B. Mustafa.Regulation of ? -Epithelial Sodium Channels (?-ENaC) by Interleukin-1 inMurine Lung Epithelial (MLE-12) Cells. J. Invest. Med, 51, S92, 2003.2. J.A. Petershack, S.R. Seidner, R. Castro, B.M. Henson, A.J. Falck, andS.B. Mustafa. Stimulation of ? -Epithelial Sodium Channels by DibucAMP inEpithelial Cells: Role of MAP Kinase Pathways. Ped. Res. 53, (4), 429A,2003.3. J. Isaac, R. J. DiGeronimo, B.M. Henson, C.E. Bishop, S.R. Seidner, andS.B. Mustafa. Mechanical Stretch regulates ? -Epithelial Na+ ChannelExpression in Murine Lung (MLE-12) Epithelial Cells: Role of MAPK Pathways.Am. J. of Resp. and Crit. Care Med., 2004.4. M.M. Vasquez, R. Castro, B.M. Henson, T. A. Biela, S.R. Seidner, andS.B. Mustafa. cAMP-Dependent Regulation of ?-ENaC in submandibular GlandEpithelial Cells: Possible Crosstalk Between PKA and PI3K SignalingPathways. J. Invest. Med. 52, S297, 2004.5. M.M. Vasquez, R. Castro, S.R. Seidner, J.A. Petershack, B.M. Henson,T.A. Biela, S.B. Mustafa. cAMP Regulation of ?-ENaC in Epithelial CellsInvolves Activation of the PI3-Kinase pathway: Role of Serum andGlucocorticoid-Induced Protein Kinase (sgk1). Ped. Res. 55, 449A, 2004.6. C.L. Blanco, J.A. Petershack, S.B. Mustafa, B.M. Henson, R.I. Clyman,S.R. Seidner. Ibuprofen increases ?-Epithelial Sodium Channel Protein inPreterm Baboons. Ped. Res. 55, 449A, 2004.7. J. Isaac, R.J. DiGeronimo, P.S. Dixon, B.M. Henson, C.E. Bishop, A.S.Nasar, S.B. Mustafa. Mechanical Stretch Regulates ?-Epithelial SodiumChannel (?-ENaC) Expression in Murine Lung Epithelial (MLE-12) Cells. J.Resp. and Crit. Care Med. 169, A737, 2004.8. J.M. King, S.B. Mustafa, P.S. Dixon, B.M. Henson, R.J. DiGeronimo.Effect of Cyclic Stretch on Tight Junction Protein Expression in Mouse LungEpithelial (MLE-12) Cells. A787, 2004.9. R.M. McAdams, P.S. Dixon, S.B. Mustafa, B.M. Henson, R.J. DiGeronimo.Cyclic stretch attenuates the effects of hyperoxia on cell proliferationand viability in pulmonary epithelial A549 cells. J. Invest. Med 53, S292,2005.10. J. Isaac, R. J. DiGeronimo, B.M. Henson, P. Dixon, S.R. Seidner, andS.B. Mustafa. Mechanical Stretch stimulates ?-Epithelial Na+ ChannelExpression in Murine Lung Epithelial Cells: Role of MAPKS. J. Invest. Med.53, S292, 2005.11. T.A. Biela, B.M. Henson, S.R. Seidner, R. Castro, and S.B. Mustafa.Synthetic Glucocorticoids (GC) Selective for Transactivation stimulate ?-ENaC Formation but do not Suppress Expression of Glucocorticoid Receptors(GCR) in Mouse Lung Epithelial (MLE-12) Cells. Ped. Res. 2005.12. M.M. Vasquez, R. Castro, S.R. Seidner, B.M. Henson, D.J. Ashton, andS.B. Mustafa. Induction of Serum and Glucocorticoid Induced Protein Kinase-1 (sgk1) and Epithelial Sodium Channels (ENaC) by cAMP in Epithelial Cells.J. Invest. Med., 54 (1), S 280, 2006.13. A.A. Reeves, S.B. Mustafa, B.M. Henson, M.M. Vasquez, and R. Castro.Trafficking and Function of the Epithelial Sodium Channel Is Inhibited ByEpidermal Growth Factor In A Rat Parotid Gland Cell Line. J. Invest. Med.,Jan 2007.14. T.A. Biela, S.R. Seidner, B.M. Henson, and S.B. Mustafa. Regulation ofepithelial sodium channels and glucocorticoid receptors by glucocorticoidsselective for transactivation. J. of Invest. Med., Jan 2008.15. T.F. Hernandez, T.A. Biela, J.A. Petershack, S.R. Seidner, B.M. Henson,and S.B. Mustafa. IL-1 transiently increases epithelial sodium channelexpression in lung epithelial cells. Ped. Res., Jan 2008.Papers1. J. A. Petershack, B.M. Henson, D. J. Ashton, S.R. Seidner, and S.B.Mustafa. Regulation of ? -Epithelial Sodium Channels by Interleukin-1 inMurine Lung Epithelial (MLE-12) Cells. (Manuscript in Preparation).2. R.M. McAdams, S.B. Mustafa, J.S. Shenberger, P.S. Dixon, B.M. Henson,and R.J. DiGeronimo. Cyclic stretch attenuates the effects of hyperoxia oncell proliferation and viability in human alveolar epithelial cells.American Journal of Physiology, Lung Cellular and Molecular Physiology,291, L166-L174, 2006.3. S.B. Mustafa, R. Castro, A.J. Falck, J.A. Petershack, B.M. Henson, Y.M.Mendoza, M.A. Choudary, and S.R. Seidner. Protein Kinase A and Mitogen-Activated Protein Kinase Pathways Mediate cAMP Induction of ?-EpithelialNa+ Channels (?-ENaC). Journal of Cellular Physiology, 215(1): 101-10,2008.4. M.M. Vasquez, R. Castro, S.R. Seidner, B.M. Henson, D.J. Ashton, andS.B. Mustafa. Induction of serum- and glucocorticoid-induced kinase-1(SGK1) by cAMP regulates increases in ?-ENaC. In Press, Journal ofCellular Physiology, 2008.5. S.B. Mustafa, J. Isaac, S.R. Seidener, P.S. Dixon, B.M. Henson, andR.J. DiGeronimo. Mechanical Stretch induces lung ?-Epithelial Na+ ChannelExpression, Experimental Lung Research, 2014.REFERENCES AVAILABLE UPON REQUEST. |
