| | | 20,000+ Fresh Resumes Monthly | |
|
|
| |
Click here or scroll down to respond to this candidateCandidate's Name , PH.D.
Street Address
PHONE NUMBER AVAILABLE, PHONE NUMBER AVAILABLE (Cell)
email: EMAIL AVAILABLE
TECHNICAL AREAS
Drug Discovery, Preclinical Toxicology, Genetic Toxicology, General Toxicology
Molecular Toxicology.
SUMMARY: STUDY DIRECTOR/RESEARCH SCIENTIST:
Performed genetic toxicology/toxicology and carcinogenesis, chemoprevention with demonstrated record of accomplishments.
Experienced with planning and executing the genetic toxicology/toxicology study projects.
Conducted GLP toxicology/genetic toxicology studies in a contract research laboratory.
Generated cost estimate for projects,
Prepared draft and final protocols, SOPs, project summaries.
Implemented regulatory compliance (GLP, FDA, OECD, EPA guidelines).
Analyzed data and interpreted study results, and prepared draft and final reports, summaries and abstracts for the sponsor and for publications in peer-reviewed journals.
Validated and implemented GLP and non-GLP genetic, cell and molecular assays,
Elucidated the mechanism of action of selected promising test chemicals
Presented experimental data at team meetings (Sponsors and the management), national and international scientific meetings/conferences.
Supervised and trained supervisor (1) and technicians (10).
Recruited technicians as and when required.
Supervised and coordinated the purchase of lab equipment and supplies.
GENETIC Toxicology:
Mutation assays:
Bacterial mutagenicity assays: Ames test (Salmonella reverse mutation assay) and E. Coli WP-2 mutagenicity assay.
Clastogenicity assays: chromosomal aberration assay, mouse and rat peripheral blood and bone marrow micronucleus assays in vitro micronucleus assay.
Mammalian gene mutation assays: Mouse lymphoma assay and V79 gene mutation assay.
Dominant lethal mutation assay.
unscheduled DNA synthesis assay
Comet assay, DNA adduct assay.
Cell transformation assay.
IN VITRO TOXICOLOGY:
Cellular assays:
Cell cytotoxicity assay (neutral red uptake assay and MTT assays).
Radioactive cell proliferation assay (tritiated thymidine DNA incorporation).
Protein synthesis assay and apoptosis assays.
Biochemical assays:
Cytochrome P-450 (isoenzymes) and b5.
Aryl hydrocarbon hydroxylase (AHH).
Glutathione S-transferases (isoenzymes) (GST).
Glutathione peroxidase (GP).
Superoxide dismutase (SOD).
Gamma-glutamyltranspeptidase (GGT).
Ornithine decarboxylase (ODC).
IMMUNOTOXICOLOGY: In vivo models of host resistance to infection, Antibody forming cells (AFC) assay, Natural killer cell (NKC) assay, T and B-cell proliferation assay, mixed lymphocyte reaction assay and quantification of cytokines (IL-1, IL-6, TNF alpha, IL-2 etc).
GENERAL TOXICOLOGY: Acute and chronic toxicity studies, Carcinogenesis, cancer chemoprevention and tissue: Specific DNA, RNA and protein detection (Southern, Northern and Western blots), Immunohistochemistry, In situ hybridization, Gel shift assays (Transcription factors - NFkB, AP-1, AP-2 and CREB), Molecular diagnosis (TaqMan, PCR, RT-PCR, SSCP, automated DNA sequencing and DNA fragment analysis.
GLP EXPERIENCE
Conducted and managed GLP and lab research for seven years; three years in a contract research laboratory and four years in clinical molecular diagnostic laboratory.
PROJECT MANAGEMENT
Generated cost estimate for projects, prepared draft and final protocols, SOPs, project summaries, implemented regulatory compliance (GLP, FDA, OECD, EPA guidelines).
Analyzed data and interpreted study results, and prepared draft, final reports, summaries and abstracts for the sponsor and for publications in peer-reviewed journals.
Validated and implemented GLP and non-GLP genetic, cell and molecular assays.
Elucidated the mechanism of action of selected promising test chemicals
Presented experimental data at team meetings (Sponsor and the management)
Recruited and supervised and trained supervisor (1) and technicians (10)
Supervised and coordinated the purchase of lab equipment and supplies.
PROFESSIONAL EXPERIENCE
2010-2011, Visiting Research Specialist, Dept. of Pharmacology, University of Illinois at Chicago, Chicago, IL
2006-2010, Research Specialist, Dept. of Surgery, Univeristy of Illinois at Chicago, Chicago, IL
2004-2006, Research Specialist, Dept. of Pathology, Loyola Univeristy Medical Center, Maywood, IL,
2001-2004, Research Scientist/Study Director, Microbiology & Molecular Biology Division, IIT Research Institute, Chicago, IL.
1997-1999: Senior Research Associate, Dept. of Microbiology/Immunology, Loyola University of Chicago, Maywood, IL,
1995-1997: Senior Research Associate, Dept. of Surgery, University of Kentucky, Lexington, KY,
1993-1995: Senior Research Associate, Institute of Pathology, Case Western Reserve University, Cleveland, OH,
1988-1993: Research Associate, Dept. of Pathology, Northwestern University, Chicago, IL,
EDUCATION
1988: Ph.D., Biochemistry, University of Bombay, Bombay, INDIA.
1980: M.S., Biochemistry, Cell & Molecular Biology, Mysore University, Mysore, INDIA.
1978: B.S., Chemistry, Zoology, Botany, Mysore University, Mysore, INDIA.
1999: Human Resources - Organizational Behavior (1 Credit), Loyola University, Chicago, Illinois, USA.
COMPUTER EXPERTISE: WinWord, Excel, PowerPoint, Cricket graph, Sigma plot.
ESTABLISHED NEW LABS/RESEARCH PROGRAMS
Genetic Toxicology Program 2
Bacterial Mutation Assay, Mouse lymphoma Assay, V79 gene mutation Assay, Cell cytotoxicity Assay, Unscheduled DNA synthesis Assay and mouse and rat peripheral blood and bone marrow micronucleus Assay.
Other Areas 3
Chemoprevention of cancer 1, Molecular Diagnosis lab 1, Transplantation Biology 1.
PUBLICATIONS:
Author or co-author of 43 publications, 89 abstracts, presentations and guest lectures.
As listed in the Medline
GENETIC TYOXICOLOGY:
1: Nagabhushan M, Line D, Polverini PJ, Solt DB. Anticarcinogenic action of diallyl sulfide in hamster buccal pouch and forestomach. Cancer Lett. 1992 Oct 21; 66(3):207-16.
2: Nagabhushan M, Bhide SV. Curcumin as an inhibitor of cancer. J Am Coll Nutr. 1992 Apr; 11(2):192-8.
3: Nagabhushan M, Sarode AV, Nair J, Amonkar AJ, D'Souza AV, Bhide SV. Mutagenicity and carcinogenicity of tea, Camellia sinensis. Indian J Exp Biol. 1991 May;29(5):401-6.
4: Nair UJ, Ammigan N, Nagabhushan M, Amonkar AJ, Bhide SV. Effect of vitamin A status of rats on metabolizing enzymes after exposure to tobacco extract or N'-nitrosonornicotine. IARC Sci Publ. 1991; (105):525-8.
5: Nagabhushan M, Ng YK, Elias R, Polverini PJ, Solt DB. Acute inhibition of DNA synthesis in hamster buccal pouch epithelium exposed to indirect acting carcinogens. Cancer Lett. 1990 Sep; 53(2-3):163-73.
6: Ammigan N, Nagabhushan M, Nair UJ, Amonkar AJ, Bhide SV. Effect of nutritional status on mutagenicity of urine excreted by rats treated with standard/experimental carcinogens. Indian J Exp Biol. 1990 Aug; 28(8):711-3.
7: Nagabhushan M, Amonkar AJ, Nair UJ, D'Souza AV, Bhide SV. Hydroxychavicol: a new anti-nitrosating phenolic compound from betel leaf. Mutagenesis. 1989 May; 4(3):200-4.
8: Sarkar S, Nagabhushan M, Soman CS, Tricker AR, Bhide SV. Mutagenicity and carcinogenicity of smoked meat from Nagaland, a region of India prone to a high incidence of nasopharyngeal cancer. Carcinogenesis. 1989 Apr; 10(4):733-6.
9: Nagabhushan M, Nair UJ, Amonkar AJ, D'Souza AV, Bhide SV. Curcumins as inhibitors of nitrosation in vitro. Mutat Res. 1988 Nov; 202(1):163-9.
10: Nagabhushan M, Bhide SV. Anti-mutagenicity of catechin against environmental mutagens. Mutagenesis. 1988 Jul; 3(4):293-6.
11: Nagabhushan M, Maru GB, Amonkar AJ, Nair UJ, Santhanam U, Ammigan N, D'Souza AV, Bhide SV. Catechin as an antimutagen: its mode of action. J Cancer Res Clin Oncol. 1988; 114(2):177-82.
12: Nagabhushan M, Amonkar AJ, Bhide SV. Mutagenicity of gingerol and shogaol and antimutagenicity of zingerone in Salmonella/microsome assay. Cancer Lett. 1987 Aug; 36(2):221-33.
13: Nagabhushan M, Amonkar AJ, Bhide SV. In vitro antimutagenicity of curcumin against environmental mutagens. Food Chem Toxicol. 1987 Jul; 25(7):545-7.
14: Chacko M, Nagabhushan M, Sarkar S, Bhide SV. Studies on the possible mutagenic action of metronidazole. Indian J Exp Biol. 1987 Apr; 25(4):240-3.
15: Nagabhushan M, Amonkar AJ, D'Souza AV, Bhide SV. Nonmutagenicity of betel leaf and its antimutagenic action against environmental mutagens. Neoplasma. 1987; 34(2):159-67.
16: Amonkar AJ, Nagabhushan M, D'Souza AV, Bhide SV. Hydroxychavicol: a new phenolic antimutagen from betel leaf. Food Chem Toxicol. 1986 Dec; 24(12):1321-4.
17: Nagabhushan M, Bhide SV. Nonmutagenicity of curcumin and its antimutagenic action versus chili and capsaicin. Nutr Cancer. 1986; 8(3):201-10.
18: Nagabhushan M, Bhide SV. Mutagenicity of chili extract and capsaicin in short-term tests. Environ Mutagen. 1985; 7(6):881-8.
TOXICOLOGY ONCOLOGY:
1. Kalaria S, Nagabhushan M, Pendyala S, Berdyshev EV, Rolle C, Kanteti R, Kanteti A, Wenli M, He D, Husain AN, Kindler HL, Prasad P, Salgia R, Natarajan V,. Sphingosine kinase I is required for meosthelioma proliferation: Role of histone acetylation. PLoS One 7: 1-30, 2012.
2. Ranjan D, Chen C, Johnston TD, Jeon H & Nagabhushan M, Curcumin inhibits mitogen stimulated lymphocyte proliferation, NfkappaB activation, and IL-2 signalling. J Surg Res 121:171-77, 2004.
3. Nagabhushan M, Mathews HL, Witek-Janusek L. Aberrant nuclear expression of AP-1 and NFkappaB in lymphocytes of women stressed by the experience of breast biopsy. Brain Behav Immun. 2001 Mar; 15(1):78-84.
4. Nagabhushan M, Pretlow TG, Guo YJ, Amini SB, Pretlow TP, Sy MS. Altered expression of CD44 in human prostate cancer during progression. Am J Clin Pathol. 1996 Nov; 106(5):647-51.
5. Nagabhushan M, Miller CM, Pretlow TP, Giaconia JM, Edgehouse NL, Schwartz S, Kung HJ, de Vere White RW, Gumerlock PH, Resnick MI, Amini SB, Pretlow TG. CWR22: the first human prostate cancer xenograft with strongly androgen-dependent and relapsed strains both in vivo and in soft agar. Cancer Res. 1996 Jul 1; 56(13):3042-6.
6. Cheng L, Nagabhushan M, Pretlow TP, Amini SB, Pretlow TG. Expression of E-cadherin in primary and metastatic prostate cancer. Am J Pathol. 1996 May; 148(5):1375-80.
7. Pretlow TG, Nagabhushan M, Pretlow TP. Prostatic intraepithelial neoplasia and other changes during promotion and progression. Pathol Res Pract. 1995 Sep; 191(9):842-9.
8. Bowie LJ, Reddy PL, Nagabhushan M, Sevigny P. Detection of alpha-thalassemias by multiplex polymerase chain reaction. Clin Chem. 1994 Dec; 40(12):2260-6.
9. Monger LE Jr, Nagabhushan M, Pretlow TG, Pretlow TP. A novel approach to the characterization of whole prostate pathology in glycol methacrylate. Am J Pathol. 1994 Jul; 145(1):54-60.
10. Pretlow TG, Nagabhushan M, Sy M, Guo Y, Pretlow TP. Putative preneoplastic foci in the human prostate. J Cell Biochem Suppl. 1994; 19:224-31.
11. Nagabhushan M, Bowie LJ, Sevigny P. A rapid procedure for microextraction of genomic DNA from whole blood for clinical diagnostic tests. Biotechniques. 1993 Nov; 15(5):824-5.
Not listed in the Medline:
1: Kolpe U, Ramaswamy V, Rao BSS, Nagabhushan M (2002) Turmeric and curcumin prevents the formation of mutagenic maillard reaction products. The Maillard reaction in food chemistry and medical science: Update for postgenomic era. Horiuchi S, Taniguchi N, Hayase F, Kurata T and Osawa T (Eds), International Congress series 1245, Elsevier Science B.V, Amsterdam, The Netherlands; pp 327-334.
2: Ranjan D, Siqijor A, Johnston TD, Wu G, Nagabhushan M. The effect of curcumin on human B-cell transformation by Epstein-Barr virus. Am. Surg. 1998; 64:47-51.
3: Nagabhushan M, Usha K, Ranjan D, Johnston TD. .Spices for the chemoprevention of gastroenterological cancers. Recent Advances in Gastroenterological Carcino-genesis I. E. Tahara, K Sugimachi, and O. Ohara (Eds.), Monduzzi Editorie, Bologna, Italy, 1996 pp. 1147-1149.
4: Nagabhushan M, Amonkar AJ, Bhide SV. Antimutagenicity of curcumins and related compounds: the structural requirement for the antimutagenicity of curcumins. Indian Drugs 1987; 21:91-95.
5: Nagabhushan M, Bhide SV. Antimutagenicity and anticarcinogenicity of turmeric (Curcuma longa). J. Nutr. Growth Cancer 1987; 4:83-89.
AWARDS:
GENETIC TOXICOLOGY:
1989: VICTOR DRILL AWARD: Society of Toxicology, Midwest Chapter, Chicago, Illinois, USA.
REFERENCES: Excellent reference will be provided upon request
|
