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| | Click here or scroll down to respond to this candidate Dr. YuHung Corey Lin
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Immigrate Status: Permanent Residence. No Requirement for sponsorship for working visa
Recruiters Summary:
Candidate's Name has Ph.D. in Chemical Biology and biophysical chemistry with experience in cell-based assays,
multiple projects based on therapeutic potential for cancer cell targets and has experience on immunology, molecular
biology; stem cells, cancer, cell purification techniques and ex vivo tissue culture. Specific experience working with novel
biomembrane mimicking substrate for potential target on cellular therapeutic for cancer cell treatments. The other
expertise is research on mechanism of the protein-protein interaction via FRET technique on novel bioassay materials,
such as solid-supported biomembrane-mimicking substrates. Extensive years of experience in immunostaining of cells
isolated from bone, muscle and cancer cells well as primary cells; analyzed cell death assays, apoptosis vs necrosis
analysis and determined the cell cycle phases of the proliferating cells. Strong Molecular Biology skills including RNA,
DNA isolations as well as analysis of gene expression using qRT-PCR, microarray analysis techniques; cell proliferation
and differentiation, transfection and transduction. He is local to Chicago IL 60640 and available for this assignment for
further notice.
Achievement towards Job Description:
Experience in Surface Plasmon Resonance instruments:
I worked on the multiple projects that were based on the therapeutic potential of the drug and cell targets.
Specific experience working with protein-protein interaction, such as mechanism of cadherin and integrin on the
cancer cell lines as well as stem cells.
Have extensive experience in the Bioassay development and have previous industry experience at BLI.
Experience in Novel Biomemebranemimicking substrates and bioassay design for cellular
mechanoresponse and proteinprotein interactions
I have extensive experience in the novel biomaterial design and manufacture
Used these techniques for phenotypic characterization of cells isolated from muscle and bone cell well as primary
cells.
Analyzed cell death assays, apoptosis vs necrosis analysis.
Determined the cell cycle phases of the proliferating cells.
Have multiple publications with cellular mechanoresponse on novel type of biomembrane mimicking
substrates reported (Lin et al 2012 and Lautschum et al, 2014).
Experience in molecular biology including transfection and viral transduction, DNA isolation, cloning, etc.:
Strong skills in molecular biology techniques such as RNA, DNA isolations and gene expression using qRT-PCR,
microarray analysis techniques.
Analysis of protein expression or quantification of secretion of cytokines or chemokines by immunocytochemistry,
western blots, ELISAs.
Experience in cell-based and protein-based immunoassays, and working with primary cells and ex- vivo analyses
is desired:
Have good hands on experience with various kinds of cells isolated from different sources such as mouse muscle
cell and cancer cells.
Extensive characterizations of the isolated cells both at transcriptional and translational levels.
Experience in post transplantation immuno-analysis, which has also been shown in one of my recent publication
(Lautschum et al, 2014, Ge et al, 2017).
Familiarity with cell purification techniques and immunological phenotyping procedures:
Experience with cell purification techniques using stable transfection and transient transfection to label cells
with specific biomarker, size exclusion membrane filtration and characterization of surface specific receptors.
Extensive experience in processing mouse cell line and immunostaining samples , isolation of mononuclear cells
and culturing to isolate different types of cells, maintenance, characterization, amplification and banking (Lautschum
et al, 2014).
Familiarity with bioanalysis techniques such as protein purification and protein-protein interactions:
Experience with HPLC and FPLC method to purify target membrane protein and retrieve from biomembrane and also
typical SDS-PAGE and other column chromatography purification.
Experience with LC-MS (Thermo Scientific Orbitrap Fusion Mass Spectrometer.) and MALDI-MS on characterize
target protein and analyze the finger-print spectrum of target protein.
Summary:
Competent, responsible and work ethic oriented professional with 6 years of experience in stem cells, cancer and
molecular biology with excellent verbal/written and analytical skills.
Passion for solving complex problems with strong managerial skills.
Skills:
Cell Biology: Cell culturing techniques (Human cancer cells, Embryonic stem cells, C2C12, fibroblast MEF and
mouse cancer cells) and Bacteria culture (Yeast, E. Coli, and Methylococcus capsulatus (Bath) (pMMO)), Transient
Transfection and stable transfection on C2C12 and MEF mouse cell line.
Biomaterials and Tissue Engineering: Fabrication of 2D biomembrane mimicking substrate and 3D-Extracellular
matrices (Electrospinning), Polymer characterization, Biomembrane-mimicking substrate design and manufacture,
Thermal analysis (TGA, DSC), NMR, Nanoparticle morphology (TEM,AFM, and Optical Electron Microscopy), pH
meter, and Viscometer.
Molecular, Protein and Biochemical Techniques: DNA and RNA Isolation, Chromatography techniques (Affinity,
HPLC, GC), AKTA, Staining techniques, Western blot, Ultra filtration, Purification methods, Cryosectioning, Confocal
imaging, Flowcytometry, ELISA, immunohistochemical staining, Agglutination, Surface Plasmon Resonance as
Biacore and BLI, and Spectrophotometry.
Bioinformatics: Molecular modeling studies: Homology modeling (SWISS), Docking, and Protein sequence analysis
tools: wide use of ExPASY web site.
Education:
Doctor of Philosophy, Purdue University, Indianapolis IN, Aug 2009 Dec 2015
Primary Area of study, Chemical Biology and Biophysical chemistry.
Masters of Science, National Tsing-Hua Univ. HsinChu Taiwan Aug 2003 - Jun 2005
Primary Area of study: Applied Chemistry, Proteomics
Bachelor of Technology, National Tsing-Hua Univ. HsinChu Taiwan Aug 1999 - Jun 2003
Primary Area of study: Chemistry
Experience:
Purdue University, Indianapolis, IN Aug 2009- Dec 2015
Graduate Research Assistant and Confocal Microscope facility manager, Chemistry Department
Designed and developed biomembrane mimicking substrate for research of cellular mechanoresponse on variant
mechanical property.
Lab/ Confocal microscope manager:
a. Training new user on Confocal Microscope facility. b. Manager Fluorescent and Confocal Microscope.
b. Strong skills in toxicity and drug testing of anticancer drugs (eg. JQ1), discography dyes, and heavy metals on
cells and analysis of their mechanisms of action via signaling pathways.
Designed experimental work flow and analysis of in vitro and translational studies using both cell lines and
animal models for cellular mechanoresponse on novel biomaterials.
Established and managed Novel Biomemebranemimicking substrates and bioassay design for
cellular mechanoresponse and proteinprotein interactions, such as Integrin and cadherin
cadherin interaction on novel materials.
Teaching assistant for General chemistry, biochemistry and analytical chemistry Lab.
Academia Sinica, Taipei, Taiwan May 2003 Jan 2006
Research Scientist, Bioprocess Process Product Development Lab
Developed and optimized upstream process including cell culture media, inoculum expansion, CHO cell culture
Lab Manager. To train undergraduate students; maintain the basic of laboratory environments.
Instrument Operator of following instruments for research and analysis
a.GCMS (Hewitt and Parkard) ; b. LCMS (Applied BioSystem); c.HPLC (Waters & HP); d. Solid
State Peptide synthesis; e. Biacore (SPR Immunoassay) f. UVVisible; g. CD; h. NMR (Bruker,
Varian); i. AA; j. PCR; k. IR; l. TEM; m. organic synthesis; n SDSPAGE o. ELISA p. other protein
purification.
Research Assistant for mechanism of protein folding on A , prion protein, and spongiform
transmissible disease.
Publications
1. Yifan Ge, Candidate's Name , Lena A. Lautscham, Wolfgang H Goldmann, Ben Fabry and Christoph A Naumann. Soft Matter,
2016 12 (40) 8259
2. Lautscham, L. A.; Lin, C. Y.; Auernheimer, V.; Naumann, C. A.; Goldmann, W. H.; Fabry, B Biomaterials 2014, 35, 3198
3. Lin, Y.-H.; Minner, D.; Herring, V.; Naumann, C. Materials 2012, 5, 2243.
4. Lawrence, K.; Dolai, S.;Lin, Y.-H.; Dass, A.; Sardar, R. RSC Advances 2014, 4, 30742
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