Title: Senior Research Associate
Duration: 12 months (Extendable)
Location: San Diego, CA 92121
Job ID: 53807-1

Pay Rate : $ 40.00 - 45.00/hr on W2

Job Description: SUMMARY:
Client (Client) seeks a highly motivated cell and molecular biology contractor to join our Oncology Discovery research team within the Oncogenesis Thematic Research Center (TRC) which specializes in protein homeostasis and targeted protein degradation. The successful candidate will work collaboratively to 1) help develop and implement cell assays to support target identification and validation;
2) conduct studies aimed at enhancing our comprehension of the on-target/off-target mechanisms of lead Client compounds; and
3) take responsibility for 2D/3D model and assay development.

The successful candidate should have strong technical skills, particularly in mammalian cell culture, CRISPR, and 3D cell models. The candidate should also possess the ability to quickly learn new protocols and effectively implement changes to enhance them. The candidate must be able to effectively demonstrate clear verbal and written communication skills. This position is based in San Diego, CA.

RESPONSIBILITIES:
Conduct target validation studies across a diverse range of oncology models, using CRISPR and tool compound approaches
Perform organoid model development and maintenance
Employ FACS and Western blots for cell analysis; ProteinSimple WES/JESS is a plus
Perform dose-response curve experiments to assess drug efficacy
Maintain and passage 2D/3D patient-derived tumor lines
Perform gene editing and various molecular techniques to characterize edited cells.
Oversee the cryopreservation of tissues and cell lines to ensure proper storage
Adhere to company regulations by meticulously maintaining precise and comprehensive laboratory notebooks and other records
Write laboratory protocols and standard operating procedures in a clear and concise manner
Perform a wide variety of basic molecular biology procedures
Assist team members with house-keeping tasks

REQUIRED SKILLS:
Solid hands-on experience with fundamental cell biology techniques such as:
o Mammalian cell culture (3D culture experience is a plus)
o Plate-based assays such as CTG, apoptosis, Incucyte
o Cryopreservation
Proficiency in essential molecular biology techniques such as:
o Transfection and viral transduction techniques (overexpression, CRISPR KO)
o Western blotting
o Flow Cytometry
Preference will be given to candidates with hands-on experience with 3D culture techniques
Attention to detail and ability to perform delicate manual tasks in a laboratory
Enthusiasm and demonstrated ability to learn and apply new laboratory techniques, skills, and instrumentations
Ability to work both independently and as part of a team
Strong communication skills (effectively conveying problems, protocols, and results to colleagues through clear written and verbal communication), and ability to maintain meticulous and comprehensive records
Strong determination to drive projects to successful completion
Able to effectively interact with colleagues of diverse backgrounds and contribute to team goals
Fluency in the use of Microsoft Word, Excel, PowerPoint, and FlowJo

EDUCATION:
M.Sc. degree in the biological sciences with 0-2 years of hands-on cell and molecular biology experience
B.Sc. degree with 2+ years of hands-on cell and molecular biology experience

Why work with us - At Net2Source, we believe everyone has an opportunity to lead. We see the importance of your perspective and your ability to create value. We want you to fit inwith an inclusive culture, focus on work-life fit and well-being, and a supportive, connected environment; but we also want you to stand outwith opportunities to have a strategic impact, innovate, and take necessary steps to make your mark. We help clients with new skilling, talent strategy, leadership development, employee experience, transformational change management and beyond.

Equal Employment Opportunity Statement: